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| Table 1. Total and CryIAb proteins rhizosecreted by transgenic maize events, and CryIAb protein content in root tissues after three weeks of hydroponic culture. |
Hybrid |
Total exudated |
CryIAb rhizosecreted |
CryIAb protein in root |
Chalten TD (176) |
16.2 ± 1.31 |
ND |
0.01 ± 0.001 |
32G63MG (MON810) |
23.7 ± 2.21 |
94 ± 5 |
1.56 ± 0.05 |
Chalten TD max (BT11) |
19.9 ± 2.03 |
19 ± 2 |
1.96 ± 0.04 |
AX82 (Non- transgenic control) |
21.19 ± 4.18 |
ND |
NA |
Three plantlets from each hybrid were sampled, and total proteins in the bag were quantified by the Bradford method. The CryIAb protein was quantified using a commercial ELISA kit (EnviroLogix) for Cry1A(b). Proper dilution of the exudated protein were prepared so that the CryIA protein fit into the calibration curve values (y = 3.2221x + 0.0015, R2 = 0.9998). CryIAb protein was also extracted and quantified from fresh root tissue. ND, not detectable; NA, not analyzed. (*) mean ± SD. |
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