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| Table 2. Total and CryIAb protein rhizosecreted by hybrid 32G63MG during 5 weeks of culture in hydroponic conditions. |
Week |
Total protein (µg / plant)(*) |
CryIAb protein (pg / plant) |
1st |
9.00 ± 1.58 |
8 ± 1 |
2nd |
23.27 ± 4.94 |
26 ± 0.1 |
3 th |
23.71 ± 2.21 |
94 ± 0.5 |
4th |
49.12 ± 2.25 |
123 ± 0.3 |
5 th |
67.42 ± 9.18 |
336 ± 0.03 |
Three plantlets were sampled at weekly intervals, and total proteins in the bag were quantified by the Bradford method. The CryIAb protein was quantified using a commercial ELISA kit (EnviroLogix) for CryIA(b). Proper dilution of the exudated protein were prepared so that the CryIA protein fit into the calibration curve values (y = 3.2221x + 0.0015, R2 = 0.9998). (*)mean ± SD. |
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