Table 3. Immunological detection of CryIAb protein in soil samples. |
Hybrid (event) |
Date |
CryIAb protein (pg / g soil)(*) |
Pucará (Control) |
February 2002 |
29 ± 3 |
May 2002 |
30 ± 2 |
|
August 2002 |
12 ± 1 |
|
November 2002 |
16 ± 3 |
|
March 2003 |
10 ± 3 |
|
Pucará TD (176) |
February 2002 |
8 ± 3 |
May 2002 |
17 ± 3 |
|
August 2002 |
3 ± 2 |
|
November 2002 |
27 ± 4 |
|
March 2003 |
21 ± 2 |
|
30F82 (control) |
February 2002 |
16 ± 2 |
May 2002 |
13 ± 3 |
|
August 2002 |
10 ± 1 |
|
November 2002 |
11 ± 3 |
|
March 2003 |
23 ± 2 |
|
32G63 MG (MON810) |
February 2002 |
16 ± 4 |
May 2002 |
9 ± 4 |
|
August 2002 |
9 ± 2 |
|
November 2002 |
4 ± 1 |
|
March 2003 |
37 ± 2 |
Three soil samples were obtained at invervals of three months. Total proteins were quantified by the Bradford method, and CryIAb protein was detected and quantified using a commercial ELISA kit (Envirologix), (*)mean ± SD. |
|