Figure 2. Doublets design and sequence.
(a) Three-primer driven PCR reaction with hybrid primer (red-green) and flanking primers (red or green). DNA templates are in red and green boxes.
(b) CEC_dir-CEC_ret (top panel) and CEC_ret-CEC_dir (bottom panel) doublets construction design: using CEC_dir (in black) and CEC_ret (in blue) as templates. Sequences of hybrid primers used for fusion are underlined in red. Initiation codon ATG is omitted from scheme.
(c) A scheme of the amino acid sequence of doublets of cecropin CEC_dir-CEC_ret (top panel) and CEC_ret-CEC_dir (bottom panel) from amino terminal to carboxi terminal orientation. Positively charged amino acids are marked by plus symbols (+). Initial methionine amino acid is omitted from scheme.