|
|
Figure 4. Expression profile of doublet. Expression of CECdir-CECret from BL21/pET cells induced with IPTG, resolved through Tris-tricine / Urea (18%) gel electrophoresis. Lane 1: Molecular Weight Ladder (Winkler Ltda, Santiago, Chile), Lane 2,3: Control (BL21 carrying pET CECdir-CECret without IPTG induction) at 37 and 26ºC 6 hrs incubation respectively, Lane 4,5: BL21 carrying pET CECdir-CECret at 37 and 26ºC with 2 hrs IPTG induction, Lane 6,7: BL21 carrying pET CECdir-CECret at 37 and 26ºC with 4 hrs IPTG induction, Lane 8,9: BL21 carrying pET CECdir-CECret at 37 and 26ºC with 6 hrs IPTG induction. |
|
|