Table 4. Mean values of enzymatic activity levels for amylases, cellulases, and acid and alkaline phosphatases determined from malt broth filtrates after culturing strains D1 and D2 of Descolea antarctica at different pH levels.


Strains

pH

Extracellular enzymes

  

  

A

B

C

D

 

4

0.120 ± 0.029 c

0.060 ± 0.000 b

1.720 ± 0.012 a

1.770 ± 0.000 a

 

5

0.245 ± 0.026 b

0.010 ± 0.000 c

0.355 ± 0.006 d

0.320 ± 0.018 e

 

6

0.680 ± 0.006 a

0.015 ± 0.003c

0.355 ± 0.006 d

0.415 ± 0.009 d

  

7

0.050 ± 0.006 d

0.005 ±0.000c

0.090 ± 0.000 f

0.090 ± 0.000 g

D1

8

0.040 ± 0.000 d

0.008 ± 0.000 c

0.150 ± 0.006 ef

0.150 ± 0.006 f

 

9

0.009 ± 0.001  d

0.005 ± 0.000 c

0.105 ± 0.003 f

0.115 ± 0.003 g

 

10

0.010 ± 0.000 d

0.009 ± 0.000 c

0.150 ± 0.000 ef

0.340 ± 0.029 e

 

11

0.003 ± 0.002  d

0.058 ± 0.002 b

1.165 ± 0.003 b

1.360 ± 0.012 b

 

*pH 5.5

0.008 ± 0.000 d

0.004 ± 0.000 c

0.350 ± 0.023 d

0.540 ± 0.000 c

 

4

0.008 ± 0.001 d

0.019 ± 0.009 c

0.015 ± 0.003 g

0.170 ± 0.000 f

 

5

0.007 ± 0.002 d

0.110 ± 0.006 a

0.180 ± 0.006 e

0.030 ± 0.000 h

 

6

0.018 ± 0.002 d

0.020 ± 0.006 c

0.135 ± 0.003 ef

0.006 ± 0.000 h

 

7

0.017 ± 0.001 d

0.012 ± 0.006 c

0.140 ± 0.017 ef

0.010 ± 0.000 h

D2

8

0.012 ± 0.004 d

0.021 ± 0.012 c

0.740 ± 0.000 c

0.015 ± 0.003 h

 

9

0.290 ± 0.007 b

0.018 ± 0.010 c

0.240 ± 0.006 de

0.020 ± 0.000 h

 

10

0.120 ± 0.006 c

0.021 ± 0.001 c

0.305 ± 0.032 d

0.105 ± 0.003g

 

11

0.040 ± 0.006 d

0.020 ± 0.000 c

0.310 ± 0.029 d

0.420 ±0.006 d

 

*pH 5.5

0.008 ± 0.001 d

0.010 ± 0.000 c

0.285 ± 0.009 d

0.140 ± 0.006 f

*pH: pH control.
A: amylases (mg glucose/ml * g of mycelium * hr).
B: cellulases (mg glucose/ml * g of mycelium * 24 hr).
C: acid phosphatases (mg p-nitrophenol/ml * g of mycelium * hr).
D: alkaline phosphatases (mg p-nitrophenol/ml * g of mycelium * hr).
Values in a column with different letters differ significantly from one another (p ≤ 0.05). n = 54.


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