Figure 2. PCR amplification of ITS gene region at different annealing temperatures.

(a) Positive control annealing at 56ºC with the primers ITS4 and ITS5.

(b), (c), (d) Annealing at 56ºC, 58ºC and 60ºC respectively with the primers BE-1 and BE-2.

M: DNA marker (DL-2000 ); N: Negative control without DNA template. The numeric codes are concordant with those in Table 1.