|
| Table 3. A comparison of mRNA recovered and number of recombinant colonies obtained from Xylem tissue of Zinfandel (PD-Susceptible) and Blanc du Bois (PD-Tolerant) infected with Pierces Disease (PD) between original and modified protocol through subtractive hybridization. |
Protocol |
Xylem Tissue |
|||
Zinfandel (Bunch type) |
Blanc du Bois (Florida hybrid) |
|||
mRNA |
No. of recombinant colonies obtained |
mRNA |
No. of recombinant colonies obtained |
|
Modified method |
3.6 |
180 |
3.2 |
140 |
Salzman method |
1.4 |
40 |
1.0 |
35 |
|
|