Figure 3. PCR analysis for selection of sense or antisense orientation of inserted genes in pHS44 (a), pHS47 (b) and pHSKAS1 (c). Lanes M: 1 kb plus DNA ladder, 1: antisense and 2: sense. Arrows and size in kb indicate the size of PCR products in sense orientation, 1.2 kb for PAT-Nos (primers TE-F and NOS-R), 1.4 kb for SAD-Nos (primers DES-F and NOS-R) and 1.7 kb for KASII-Nos (primers KAS-F and NOS-R).