Figure 5. Physical map and restriction endonuclease analysis of pHS48 (13618 bp). The double heads dash arrows indicate the size of fragments digested with NheI, (a) 2627 bp (5761-3134) and (b) 2496 bp (8257-5761). The double head arrows indicate the size of fragments digested with AscI, (c) 909 bp (7009-6100), (d) 1587 bp (8596-7009) and (e) 1116 bp (9712-8596). Equal amount of DNA of pHS48 was digested with different restriction endonucleases (Lanes 1: AscI, 2: AvrII, 3: BamHI, 4: BglII, 5: EcoRI, 6: EcoRV, 7: HindIII, 8: NheI, 9: PstI, 10: SalI, 11: SpeI and 12: XhoI). The size of the observed fragments in each digestion was as predicted. Red arrows indicate the size of fragments digested with AscI and NheI as predicted in physical map. Lane M is 1 kb plus DNA ladder.