Figure 6. Physical map and restriction endonuclease analysis of pHS50 (13978 bp). The double heads dash arrows indicate the size of fragments digested with NheI, a) 2124 bp (6204-4080) and b) 2627 bp (8831-6204). The double head arrows indicate the size of fragments digested with AscI, c) 1468 bp (3741-2273), d) 5429 bp (9170-3741) and e) 909 bp (10079-9170). Equal amount of DNA was digested with different restriction endonucleases (Lanes 1: AscI, 2: AvrII, 3: BamHI, 4: BglII, 5: EcoRI, 6: EcoRV, 7: HindIII, 8: NheI, 9: PstI, 10: SalI, 11: SpeI and 12: XhoI). The size of the observed fragments in each digestion was as predicted. Red arrows indicate the size of fragments digested with AscI and NheI as predicted in physical map. Lane M is 1 kb plus DNA ladder.


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