Figure 7. Physical map and restriction endonuclease analysis of pHS51 (16688 bp). The double heads dash arrows indicate the size of fragments digested with NheI, a) 3236 bp (6204-2968), b) 2627 bp (8831-6204) and c) 2496 bp (11327-8831). The double head arrows indicate the size of fragments digested with AscI, d) 1468 bp (4775-3307), e) 4395 bp (9170-4775), f) 909 bp (10079-9170), g) 1587 bp (11666-10079) and h) 1116 bp (12782-11666). Equal amount of DNA was digested with different restriction endonucleases (Lanes 1: AscI, 2: AvrII, 3: BamHI, 4: BglII, 5: EcoRI, 6: EcoRV, 7: HindIII, 8: NheI, 9: PstI, 10: SalI, 11: SpeI and 12: XhoI). The size of the observed fragments in each digestion was as predicted. Red arrows indicate the size of fragments digested with AscI and NheI as predicted in physical map. Lane M is 1 kb plus DNA ladder.