|
| Table 1. RAPD primers used to detect polymorphism, number of bands for polymorphism between landraces per primer. |
RAPD Primer |
Sequence (5’-3’) |
Number of bands |
Number of polymorphic markers |
Percentage |
PIC value |
OPC9 |
CTCACCGTCC |
2 |
2 |
100.00 |
0.353 |
OPC10 |
TGTCTGGGTG |
8 |
4 |
50.00 |
0.283 |
OPC11 |
AAAGCTGCGG |
8 |
3 |
37.50 |
0.122 |
OPC12 |
TGTCATCCCC |
20 |
10 |
50.00 |
0.184 |
OPC13 |
AAGCCTCGTC |
8 |
5 |
62.50 |
0.253 |
OPC14 |
TGCGTGCTTG |
11 |
2 |
18.18 |
0.045 |
OPC15 |
GACGGATCAG |
8 |
6 |
75.00 |
0.313 |
OPC16 |
CACACTCCAG |
9 |
6 |
66.67 |
0.255 |
OPC17 |
TTCCCCCCAG |
14 |
8 |
57.14 |
0.162 |
OPC18 |
TGAGTGGGTG |
8 |
5 |
62.50 |
0.240 |
OPC19 |
GTTGCCAGCC |
14 |
7 |
50.00 |
0.146 |
OPC20 |
ACTTCGCCAC |
16 |
7 |
43.75 |
0.137 |
OPH12 |
ACGCGCATGT |
10 |
7 |
70.00 |
0.242 |
OPH14 |
ACCAGGTTGG |
17 |
16 |
94.12 |
0.287 |
OPH17 |
CACTCTCCTC |
2 |
0 |
0.00 |
0.180 |
OPH19 |
CTGACCAG.CC |
10 |
9 |
90.00 |
0.404 |
OPH20 |
GG.GAGACATC |
16 |
3 |
18.75 |
0.053 |
OPM1 |
GTTGGTGGCT |
10 |
8 |
80.00 |
0.228 |
OPM2 |
ACAACGCCTC |
14 |
10 |
71.43 |
0.242 |
OPM4 |
GGCGGTTGTC |
8 |
6 |
75.00 |
0.323 |
OPM5 |
GGGAACGTGT |
5 |
4 |
80.00 |
0.396 |
OPM6 |
CTGGGCAACT |
17 |
16 |
94.12 |
0.300 |
OPM7 |
CCGTGACTCA |
7 |
7 |
100.00 |
0.254 |
OPM8 |
TCTGTTCCCC |
16 |
16 |
100.00 |
0.436 |
OPM9 |
GTCTTGCGGA |
24 |
24 |
100.00 |
0.283 |
OPM10 |
TCTGGCGCAC |
15 |
13 |
86.67 |
0.251 |
OPO2 |
ACGTAGCGTC |
22 |
19 |
86.36 |
0.306 |
OPO3 |
CTGTTGCTAC |
15 |
15 |
100.00 |
0.369 |
OPO4 |
AAGTCCGCTC |
18 |
16 |
88.89 |
0.354 |
OPO5 |
CCCAGTCACT |
11 |
7 |
63.64 |
0.167 |
OPO8 |
CCTCCAGTGT |
7 |
5 |
71.43 |
0.240 |
OPO10 |
TCCCACGCAA |
31 |
31 |
100.00 |
0.269 |
OPO11 |
CCTCCAGTGT |
7 |
6 |
85.71 |
0.333 |
OPO13 |
GACAGGAGGT |
12 |
12 |
100.00 |
0.305 |
OPQ1 |
GGGACGATGG |
17 |
4 |
23.53 |
0.082 |
OPQ2 |
TCTGTCGGTC |
14 |
13 |
92.86 |
0.354 |
OPQ3 |
GGTCACCTCA |
14 |
10 |
71.43 |
0.169 |
OPQ4 |
AGTGCGCTGA |
17 |
13 |
76.47 |
0.193 |
OPQ5 |
CCGCGTCTTG |
14 |
11 |
78.57 |
0.227 |
OPQ6 |
GAGCGCCTTG |
15 |
12 |
80.00 |
0.189 |
OPQ7 |
CCCCGATGGT |
9 |
8 |
88.89 |
0.263 |
OPQ9 |
GGCTAACCGA |
22 |
19 |
86.36 |
0.223 |
OPQ10 |
TGTGCCCGAA |
12 |
7 |
58.33 |
0.193 |
OPY9 |
GCAGCGCAC |
14 |
10 |
71.43 |
0.239 |
OPZ1 |
TCTGTGCCAC |
13 |
8 |
61.54 |
0.118 |
OPZ05 |
TCCCATGCTG |
12 |
10 |
83.33 |
0.246 |
OPZ08 |
TCCCATGCTG |
14 |
9 |
64.29 |
0.203 |
OPZ11 |
CTCAGTCGCA |
16 |
15 |
93.75 |
0.280 |
OPZ12 |
TCAACGGGAC |
12 |
3 |
25.00 |
0.065 |
OPZ13 |
GACTAAGCCC |
1 |
0 |
0.00 |
0.260 |
OPZ16 |
TCCCCATCAC |
12 |
6 |
50.00 |
0.142 |
OPBA13 |
AGGGCGAATG |
17 |
8 |
47.06 |
0.266 |
OPBA15 |
GAAGACCTGG |
13 |
11 |
84.62 |
0.212 |
OPBA16 |
CCACGCATCA |
17 |
8 |
47.06 |
0.121 |
OPBB1 |
ACACTGGCTG |
18 |
7 |
38.89 |
0.120 |
OPBB5 |
GGGCCGAACA |
7 |
5 |
71.43 |
0.317 |
OPBB6 |
CTGAAGCTGG |
18 |
15 |
83.33 |
0.277 |
OPBB7 |
GAAGGCTGGG |
18 |
11 |
61.11 |
0.350 |
OPBB9 |
AGGCCGGTCA |
9 |
4 |
44.44 |
0.122 |
OPBB11 |
TGCGGGTTCC |
16 |
11 |
68.75 |
0.259 |
OPBB13 |
CTTCGGTGTG |
14 |
12 |
85.71 |
0.233 |
OPBB14 |
GTGGGACCTG |
7 |
5 |
71.43 |
0.300 |
OPBC2 |
ACAGTAGCGG |
15 |
15 |
100.00 |
0.448 |
OPBC3 |
GGCTTGACCT |
10 |
8 |
80.00 |
0.422 |
OPBC4 |
CCACGTGCCA |
15 |
6 |
40.00 |
0.149 |
OPBC10 |
AACGTCGAGG |
16 |
14 |
87.50 |
0.304 |
OPBC20 |
AGCACTGGGG |
15 |
15 |
100.00 |
0.219 |
OPBD9 |
CCACGGTCAG |
19 |
11 |
57.89 |
0.180 |
OPBD10 |
CTGCTGGGAC |
18 |
17 |
94.44 |
0.257 |
OPBD16 |
GAACTCCCAG |
11 |
8 |
72.73 |
0.273 |
OPBD17 |
GTTCGCTCCC |
16 |
12 |
75.00 |
0.245 |
OPBD18 |
ACGCACACTC |
18 |
18 |
100.00 |
0.298 |
OPBD19 |
GGTTCCTCTC |
14 |
11 |
78.57 |
0.271 |
OPBD20 |
AGGCGGCACA |
18 |
14 |
77.78 |
0.194 |
|
Total |
987 |
719 |
|
|
|
Average per primer |
13.35 |
|
70.30 |
0.243 |
|
|