|
| Table 2. ISSR primers used to detect polymorphism, number of bands for polymorphism between landraces per primer. |
ISSR Primer |
Sequence (5’-3’) |
Number of bands |
Number of polymorphic markers |
Percentage polymorphism |
PIC value |
UBC807 |
AGAGAGAGAGAGAGAGT |
16 |
2 |
12.50 |
0.041 |
UBC808 |
AGAGAGAGAGAGAGAGC |
10 |
7 |
70.00 |
0.260 |
UBC810 |
GAGAGAGAGAGAGAGAT |
3 |
2 |
66.67 |
0.200 |
UBC811 |
GAGAGAGAGAGAGAGAC |
6 |
1 |
16.67 |
0.080 |
UBC814 |
CTCTCTCTCTCTCTCTA |
6 |
2 |
33.33 |
0.160 |
UBC816 |
CACACACACACACACAT |
11 |
6 |
54.55 |
0.247 |
UBC820 |
GTGTGTGTGTGTGTGTC |
10 |
10 |
100.00 |
0.296 |
UBC821 |
GTGTGTGTGTGTGTGTC |
19 |
12 |
63.16 |
0.404 |
UBC824 |
TCTCTCTCTCTCTCTCG |
8 |
8 |
100.00 |
0.308 |
UBC826 |
ACACACACACACACACC |
12 |
3 |
25.00 |
0.097 |
UBC827 |
ACACACACACACACACG |
12 |
3 |
25.00 |
0.045 |
UBC828 |
TGTGTGTGTGTGTGTGA |
5 |
0 |
0.00 |
0.000 |
UBC829 |
TGTGTGTGTGTGTGTGC |
13 |
5 |
38.46 |
0.131 |
UBC834 |
AGAGAGAGAGAGAGAGYT |
26 |
6 |
23.08 |
0.058 |
UBC836 |
AGAGAGAGAGAGAGAGYA |
8 |
6 |
75.00 |
0.135 |
UBC840 |
GAGAGAGAGAGAGAGAYT |
16 |
2 |
12.50 |
0.043 |
UBC841 |
GAGAGAGAGAGAGAGAYC |
16 |
11 |
68.75 |
0.233 |
UBC843 |
CTCTCTCTCTCTCTCTRA |
18 |
17 |
94.44 |
0.377 |
UBC844 |
CTCTCTCTCTCTCTCTRC |
17 |
16 |
94.12 |
0.374 |
UBC846 |
CACACACACACACACART |
12 |
5 |
41.67 |
0.127 |
UBC849 |
GTGTGTGTGTGTGTGTYA |
10 |
10 |
100.00 |
0.296 |
UBC851 |
GTGTGTGTGTGTGTGTYG |
5 |
3 |
60.00 |
0.332 |
UBC852 |
TCTCTCTCTCTCTCTCRA |
4 |
2 |
50.00 |
0.125 |
UBC853 |
TCTCTCTCTCTCTCTCRT |
9 |
4 |
44.44 |
0.149 |
UBC854 |
TCTCTCTCTCTCTCTCRG |
20 |
19 |
95.00 |
0.259 |
UBC855 |
ACACACACACACACACYT |
24 |
23 |
95.83 |
0.253 |
UBC857 |
ACACACACACACACACYG |
18 |
13 |
72.22 |
0.250 |
UBC859 |
TGTGTGTGTGTGTGTGRC |
15 |
13 |
86.67 |
0.325 |
UBC862 |
AGCAGCAGCAGCAGCAGC |
13 |
12 |
92.31 |
0.311 |
UBC864 |
ATGATGATGATGATGATG |
20 |
19 |
95.00 |
0.287 |
UBC867 |
GGCGGCGGCGGCGGCGGC |
5 |
5 |
100.00 |
0.264 |
UBC873 |
GACAGACAGACAGAC A |
9 |
0 |
0.00 |
0.000 |
UBC880 |
GGAGAGGAGAGGAGA |
23 |
19 |
82.61 |
0.298 |
UBC884 |
HBHAGAGAGAGAGAGAG |
14 |
3 |
21.43 |
0.059 |
UBC885 |
HBHAGAGAGAGAGAGAG |
13 |
7 |
53.85 |
0.168 |
UBC886 |
VDVCTCTCTCTCTCTCT |
18 |
9 |
50.00 |
0.267 |
UBC895 |
AGGTCGCGGCCGCNNNNNNAT |
15 |
11 |
73.33 |
0.240 |
|
Total |
479 |
296 |
|
|
|
Average per primer |
12.94 |
|
61.79 |
0.203 |
Single
letter abbreviations for mixed base positions |
|
|