Microbial Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 11 No. 4, Issue of October 15, 2008
© 2008 by Pontificia Universidad Católica de Valparaíso -- Chile Received June 5, 2007 / Accepted September 27, 2007
DOI: 10.2225/vol11-issue4-fulltext-1

Equipment design for biosorption studies with microorganisms

Thomas E. Jensen
Department of Biological Sciences
Lehman College
City University of New York
250 Bedford Park Boulevard West, Bronx
NY 10468, USA
Tel: 718 960 8235
Fax: 718 960 8236
E-mail: dolores.vitanza@lehman.cuny.edu

Mary C. Crang
Trauma Unit
Loyola University Medical Center
2160 S. First Ave., Maywood
IL 60153, USA
Tel: 708 327 3717
Fax: 708 327 2818
E-mail: mhitt@lumc.edu

Richard F.E. Crang*
Department of Plant Biology
University of Illinois at Urbana-Champaign
505 S. Goodwin Ave., Urbana
IL 61801, USA
Tel: 217 333 0616
Fax: 217 244 7246
E-mail: r-crang@life.uiuc.edu

*Corresponding author

Financial support: This work was funded in part by a grant from the Research Foundation of the City University of New York and NIH SCORE grant #5S06GM008225-22.

Keywords: bead maker, biosorption, flow-cell, sodium alginate.


ICP: Inductively Coupled Plasma

Abstract   Full Text

Two laboratory devices have been designed for experimental use in biosorption studies involving the uptake and controlled release of elements from encapsulated living cells of microorganisms. The first device is an alginate bead maker capable of producing uniform (1.5 mm diameter) sodium alginate beads with encapsulated microorganisms. The second device is a flow-cell that can subject the encapsulated microorganisms to changing fluids, streaming gaseous microaerophyllic conditions, and which also allows for samples of fluid and beads to be extracted at any time during changing experimental conditions. Both devices are novel and simple in their design, and enable improved accuracy and precise handling of encapsulated specimens with minimal labour and expenditure.

Supported by UNESCO / MIRCEN network