Biocatalytic oxidative kinetic resolution of (±)-4-(chlorophenyl)phenylmethanol by Nocardia corallina B-276
Mario A. Ramírez
Herminia I. Pérez*
Financial support: Consejo Nacional de Ciencia y Tecnología (CONACyT), México, Grant No.37272-N and Universidad Autónoma Metropolitana, México.
Keywords: 4-chlorobenzophenone, biocatalysis, microbial oxidation, resolution.
Chiral diarylmethanols are versatile building blocks for the preparation of biologically active substances, but they are difficult to obtain in enantiopure form. We used Nocardia corallina B-276 for the oxidative kinetic resolution of (±)-4-(chlorophenyl)phenylmethanol, 1. Two experimental methods were used: 1) Suspension of cells in a phosphate buffer solution and 2) Cells in the culture media, in a 3-L bioreactor. After 36 hrs using the first method, the ketone/alcohol ratio was 56/44 and the unoxidized alcohol had an enantiomeric ratio of 93/7, predominating the R-alcohol.