Figure 5. Analysis of affinity purified recombinant Liv-MIH-1 protein by Coomassie-stained-Tricine-SDS-PAGE. M: SeeBlue Pre-Stained Standard (Invitrogen). Lane 1 corresponds to the non-purified protein; Lanes 2 to 4 correspond to the samples obtained from the first three serial washes of the protein-bound resin; Lanes 5 to 7 correspond to the three serial elutions of Liv-MIH-1.