Figure 6. Analysis of enterokinase-treated Liv-MIH-1 recombinant protein by Coomassie-stained-Tricine-SDS-PAGE. M: MultiMark Multicolored Standard (Invitrogen). Lane 1 corresponds to the undigested purified protein; Lanes 2 to 4 correspond to the purified protein digested with enterokinase: Lane 2, 0.1 U; Lane 3, 0.5 U and Lane 4, 1 U.