Figure 3. SDS-PAGE of GF-Spr1 at various stages of purification. Lane-M, Molecular weight marker; Lane-1, Insoluble fraction of crude extract; Lane-2, Soluble fraction of crude extract; Lane-3, Ni-NTA purified enzyme. The pro-protein (40 kDa), mature protein (30 kDa), and pro-peptide (10 kDa) are indicated by arrows.


Supported by UNESCO / MIRCEN network