Figure 4 - Molecular cloning, expression and characterization of a serine proteinase from Japanese edible mushroom, Grifola frondosa: solving the structure - function anomaly of a reported aminopeptidase

Figure 4. Refolding and the auto processing of pro-GF-Spr1. The two stage refolding method (Marston, 1986; Tang et al. 2002) was employed for refolding of the insoluble pro-form of the enzyme as described in Experimental Procedures section. Lane-M, Molecular weight marker; Lane-1, Refolded pro-GF-Spr1; Lane-2, Auto-processed GF-Spr1. PE stands for pro-enzyme Pro-GF-Spr1; MP stands for mature protein GF-Spr1; PP stands for N-terminal pro-peptide.