Plant Biotechnology
 

Molecular Biology and Genetics

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 11 No. 5, Special Issue, 2008
© 2008 by Pontificia Universidad Católica de Valparaíso -- Chile     
DOI: 10.2225/vol11-issue5-fulltext-1
RESEARCH ARTICLE

Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars

Gabriela Rojas
Laboratorio de Biotecnología
INIA CRI La Platina
Av. Santa Rosa #11610, La Pintana
Santiago, Chile
Tel: 56 2 7575129
Fax: 56 2 7575139
E-mail: grojas@inia.cl

Marco A. Méndez
Laboratorio de Genómica Evolutiva
INTA – Universidad de Chile.
Av. Macul #5540, Macul
Santiago, Chile
Tel: 56 2 9781552
Fax: 56 2 2214030
E-mail: mmendez@inta.cl

Carlos Muñoz
Departamento de Fitomejoramiento y Biotecnología
INIA CRI La Platina
Av. Santa Rosa #11610, La Pintana
Santiago, Chile
Tel: 56 2 7575157
Fax: 56 2 7575139
E-mail: cmunoz@inia.cl

Gamalier Lemus
INIA CRI La Platina
Av. Santa Rosa #11610, La Pintana
Santiago, Chile
Tel: 56 2 7575143
Fax: 56 2 7575139
E-mail: glemus@inia.cl

Patricio Hinrichsen*
Laboratorio de Biotecnología
INIA CRI La Platina
Av. Santa Rosa #11610, La Pintana
Santiago, Chile
Tel: 56 2 7575110
Fax: 56 2 7575139
E-mail: phinrichsen@inia.cl

*Corresponding author

Financial support: Financed by FONDEF-Chile, Grant D04I-1060.

Keywords:cultivar identification, fingerprinting, Prunus persica, simple sequence repeats, SSR.

Abbreviations:

AFLP: amplified length polymorphism
IP: intellectual property
PCR: polymerase chain reaction
S-SAP: sequence-specific amplification polymorphism
SSR: simple sequence repeats

Abstract   Full Text

The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars under study could be identified using only 7 SSRs. Other 5 cultivars were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average expected heterozygosity for the set of markers was 0.55, ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022, with an F value of 0.37. A Neighbor-Joining dendrogram showed that, with few exceptions, peaches and nectarines clustered separately. These results are the basis for the development of a fingerprinting protocol for the unequivocal identification of most of the peach and nectarine cultivars officially registered in Chile.

Supported by UNESCO / MIRCEN network