Process Biotechnology

Plant Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 11 No. 5, Special Issue, 2008
© 2008 by Pontificia Universidad Católica de Valparaíso -- Chile  
DOI: 10.2225/vol11-issue5-fulltext-3

Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants

Maribel Ramírez-Villalobos*
Laboratorio de Cultivo de Tejidos
Instituto de Investigaciones Agronómicas
Departamento de Botánica
Facultad de Agronomía
Universidad del Zulia
A.P. 15205, ZU4005, Venezuela
Tel: 58 261 7596184
Fax: 58 261 7596184

Eva de García
Laboratorio de Biotecnología Vegetal
Centro de Botánica Tropical
Instituto de Biología Experimental
Facultad de Ciencias
Universidad Central de Venezuela
Los Chaguaramos, Caracas 1041, Venezuela
Tel: 58 261 7596184
Fax: 58 261 7596184

*Corresponding author

Financial support: Fundación UCV (FUCV) through the Project No. 0297/2006 and Academic Vicerectorate of the University del Zulia, for the Grant for Graduate Studies in Botany, Facultad de Ciencias, Universidad Central de Venezuela.

Keywords: bananas, embryogenic callus, embryogenic cell suspension, Musa sp, plant regeneration, scalp, secondary somatic embryogenesis.


2.4-D: 2.4-dichlorophenoxiacetic acid
BA: benzyladenine
CE: conversion of embryos
CNEC: compact non-embryogenic calluses
ECS: embryogenic cell suspension
ES: explants with scalps
IAA: indoleacetic acid
ME: mature embryos
MM: maturation medium
MS: Murashige and Skoog medium
NECS: non-embryogenic cell suspension
NNEC: nodular non-embryogenic calluses
NS: number of scalps cm-2 of the multiple meristem mass.
PSE: primary somatic embryos
SCV: sedimented cell volumen
VP: percentages of vitroplants thus obtained.

Abstract   Full Text

The purposes of this work were to obtain embryogenic cell suspensions (ECS) from scalps and to regenerate plants of the banana CIEN-BTA-03. Shoot apexes were grown in the scalp-induction medium of Murashige and Skoog plus BA and IAA, following four diverse treatments. The first two, ME22 and ME25, were solid media supplemented with (mg L-1) 22.7 BA plus 0.192 IAA, and 25 BA plus 0.217 IAA, respectively, all containing 1.8 g L-1 of phytagel, and subcultures were performed monthly and bimonthly over 16 months. The other two treatments, IT22 and IT25, resembled ME22 and ME25 but consisted in temporary immersion for four months without subcultures, followed by two months in solid media. The scalps were grown in callus-induction medium and embryogenic calluses were obtained with abundant somatic embryos, especially in scalps from IT25. About 10 to 15 embryos from each were transferred to 5 ml of multiplication medium to initiate the ECS. The scalps obtained from the IT25 treatment were the most successful as they led to ECS with high embryogenic capability. In addition, IT25 decreased the timespan required for the production of scalps. The obtained ECS gave rise to secondary somatic embryos. It showed a high multiplication index, as well as numerous mature somatic embryos, and good conversion of embryos and plant regeneration.

Supported by UNESCO / MIRCEN network