Microbial Biotechnology
 

Environmental Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 11 No. 5, Special Issue, 2008
© 2008 by Pontificia Universidad Católica de Valparaíso -- Chile  
DOI: 10.2225/vol11-issue5-fulltext-4
RESEARCH ARTICLE

Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation

Marcela Hernández García
Programa de Doctorado en Ciencias de Recursos Naturales
Universidad de La Frontera, Temuco
Laboratorio de Microbiología Molecular y Biotecnología Ambiental
Millennium Nucleus of Microbial Ecology and
Environmental Microbiology and Biotechnology
Departamento de Química
Universidad Técnica Federico Santa María
Av. España 1680, Valparaíso, Chile
Tel: 56 32 2654236
Fax: 56 32 2654782
E-mail: mthernan@ufro.cl

Verónica Magalí Morgante
Laboratorio de Microbiología Molecular y Biotecnología Ambiental
Millennium Nucleus of Microbial Ecology and
Environmental Microbiology and Biotechnology
Departamento de Química
Universidad Técnica Federico Santa María
Av. España 1680, Valparaíso, Chile
Tel: 56 32 2654236
Fax: 56 32 2654782
E-mail: veronica.morgante@gmail.com

Marcela Ávila Perez#
Laboratorio de Microbiología Molecular y Biotecnología Ambiental
Millennium Nucleus of Microbial Ecology and
Environmental Microbiology and Biotechnology
Departamento de Química
Universidad Técnica Federico Santa María
Av. España 1680, Valparaíso, Chile
Tel: 56 32 2654236
Fax: 56 32 2654782
E-mail: avila@science.uva.nl

Patricio Villalobos Biaggini
Laboratorio de Microbiología Molecular y Biotecnología Ambiental
Millennium Nucleus of Microbial Ecology and
Environmental Microbiology and Biotechnology
Departamento de Química
Universidad Técnica Federico Santa María
Av. España 1680, Valparaíso, Chile
Tel: 56 32 2654236
Fax: 56 32 2654782
E-mail: patricio.villalobos@usm.cl

Pola Miralles Noé
Laboratorio de Microbiología Molecular y Biotecnología Ambiental
Millennium Nucleus of Microbial Ecology and
Environmental Microbiology and Biotechnology
Departamento de Química
Universidad Técnica Federico Santa María
Av. España 1680, Valparaíso, Chile
Tel: 56 32 2654236
Fax: 56 32 2654782
E-mail: pola_miralles@yahoo.es

Myriam González Vergara
Laboratorio de Microbiología Molecular y Biotecnología Ambiental
Millennium Nucleus of Microbial Ecology and
Environmental Microbiology and Biotechnology
Departamento de Química
Universidad Técnica Federico Santa María
Av. España 1680, Valparaíso, Chile
Tel: 56 32 2654236
Fax: 56 32 2654782
E-mail: myriam.gonzalez@usm.cl

Michael Seeger Pfeiffer*
Laboratorio de Microbiología Molecular y Biotecnología Ambiental
Millennium Nucleus of Microbial Ecology and
Environmental Microbiology and Biotechnology
Departamento de Química
Universidad Técnica Federico Santa María
Av. España 1680, Valparaíso, Chile
Tel: 56 32 2654236
Fax: 56 32 2654782
E-mail: michael.seeger@usm.cl

*Corresponding author

Present address: #Laboratory for Molecular Microbial Physiology. Swammerdam Institute for Life Sciences, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands, Phone: 31 20 5257053, Fax: 31 20 5257056, E-mail: avila@science.uva.nl

Financial support: MS acknowledges financial support from Millennium Nucleus EMBA P04/007-F, ICA4-CT-2002-10011 (European Union), USM 130522 and USM 130836 grants. MH, VM and PV acknowledge CONICYT and MECESUP-UCV-0206 fellowships.

Keywords: Arthrobacter, biodegradation, bioremediation, simazine, Stenotrophomonas, s-triazine.

Abbreviations:

AM: atrazine medium
ARDRA: amplified ribosomal DNA restriction analysis
BSA: bovine serum albumin
CFU: colony forming unit
LB: luria bertani
PCR: polymerase chain reaction
TBE: tris borate EDTA
TSA: tryptic soy agar

Abstract   Full Text

s-Triazine-degrading bacterial strains were isolated from long-term simazine-treated agricultural soils of central Chile. The total heterotrophic bacteria of these agricultural soils (7 x 106 CFU/g of dry soil) were not affected after simazine application on field. By enrichment in minimal medium containing simazine as the sole nitrogen source, the bacterial strains P51, P52 and C53 were isolated and selected based on their simazine-degrading abilities. Resting cells of strains P51 and P52 degraded >80% of simazine within 48 hrs, whereas strain C53 was able to remove >60% of the herbicide. The atzA and atzD genes of the s-triazine catabolic pathways were detected in strains P51 and C53, while only atzD gene was observed in strain P52. To compare the bacterial 16S rRNA gene restriction pattern, ARDRA were performed using the restriction enzymes Msp1 and Hha1. ARDRA indicate that strain P52 is a different ribotype that C53 and P51 strains. For further characterization, the novel isolates were identified by 16S rRNA gene sequencing. Strains C53 and P51 belong to the genus Stenotrophomonas and the strain P52 belongs to the genus Arthrobacter. s-Triazine-degrading bacteria isolated from contaminated soils could be used as biocatalysts for bioremediation of these herbicides.

Supported by UNESCO / MIRCEN network