Figure 1. Constructions used for the transformation with antifungal genes. a) and b) Double expression cassettes containing AP24 and chitinase (CHI) genes or CHI and glucanase (GLU) genes respectively. c) Polyprotein construction containing genes of interest (CHI, GLU, NIA protease, AP24, and the Ribosome Inhibitor Protein (RIP). d) Polyprotein construction containing GUS and GFP reporter genes and NIA protease gene.

35S: CaMV35S promoter; W: 65-bp leader synthetic sequence from TMV; Nt, nopaline sintase terminator from A. tumefaciens; : NIA protease recognition site.