Figure 1. Somatic embryogenesis derived from scalps of the banana CIEN-BTA-03.
(a) Temporary immersion system.
(b-c) Multiple meristems at the base of the shoots after 4 months in (a).
(d-e) Multiple small meristems (1-3 mm, arrow) coming from (b) and after two months in ME25.
(f-g) Tissue features after 16 months in ME22 or ME25; note the formation of multiple meristems of diverse sizes.
(h-i) Non-embryogenic calluses; nodular ones were frequent in all explants whereas compact ones were rare after 5 months.
(j-k) Primary somatic embryos (SE) developed from scalps from TI25, at 3.5 months.
(l-m) Embryogenic callus with numerous SE (arrow) on its periphery, adequate for the establishment of ECS.
(n) Predominant embryonic cells aggregate in the ECS, at two months.
(ñ-o) Embryogenic cell.
(p) Cell aggregate consisting of parenchymatous cells with starch granules, highly frequent in NECS, at two months.
(q) Non-embryogenic cells in NECS, at 16 days.
(r) ECS, at four months.
(s-u) SE in maturation-medium, at 20 days (s-t) and 30 days (u).
(v-z) SE in embryo-conversion medium, at one month.
(aa-ab) Regenerated plantlet in MS plus 400 mg L^-1 of BA, at 21 days and 1.5 months, respectively, coming from (U).
(a) Bar = 45 mm.
(b, f, l, s, ab) Bar = 10 mm.
(c, h, aa) Bar = 5 mm.
(d, i-k, m) Bar = 1 mm.
(e, g, t-u) Bar = 2 mm.
(n, p-q) Bar = 100 µm.
(ñ-o) Bar = 50 µm.
(r) Bar = 22 mm.
(v-z) Bar = 2,5 mm.