Figure 1. Developmental assessment of transgenic
cloned embryos generated with bovine embryonic fibroblasts (BEF) expressing
HcRed.
a) Transfected BEF expressing HcRed (20x);
b) Expression of the red fluorescent protein in the
cellular nuclei (40x);
c) Karyotyping analysis of metaphase spreads of
transfected BEF, with sexual chromosomes highlighted (arrows);
d) Nuclear transfer procedure with cells expressing
HcRed;
e-h) Developmental assessment of transgenic cloned
embryo expressing the red fluorescent protein in the blastomeres (HcRed). |