Table 1. Primer sequences used to detect simultaneously 11 viruses by means of RT-PCR.


Virus

Acronym

Forward primer (5’-3’)

Reverse primer (5’-3’)

Product size (bp)

Arabis Mosaic Virus

ArMV

GCCCACGACTTTTCTAATCAAGA

CTAAACGCCTGTCGGAGTAATAA

295

Grapevine leafroll associated virus 1

GLRaV1

TTCAGCTTACTGTGGTCTCTATGG

GCTTCATTTGGAGTCACGAATG

109

Grapevine leafroll associated virus 2

GLRaV2

TACACCTCTCGCAATGTTTATCAC

GACCGCTAATCTTCACCGTTAT

139

Grapevine leafroll associated virus 3

GLRaV3

TAACCACCATGAAGTTCATAGGG

GCATTAGAAACAGCTTCGAATACGA

274

Grapevine leafroll associated virus 8

GLRaV8

AGAAGAGAGGGATTGGATTGGAAT

AGTCTTCTCGGTGGCTTAAATTC

172

Grapevine fanleaf virus

GFLV

TTCTTTACTTGCCCTTATGGGC

CAAAATGCCAGTCGCATTTCTC

254

Grapevine Fleck virus

GFkV

TTCACAACACAATCCAGAAGGATAC

CTCTTCATGAACATGACCGTGG

262

Grapevine rootstock stem lesion associated virus

GRSLaV

CAATTGAGTTTTGGTCAGCCTG

ACGGCAAAAATACCATTCACAC

317

Grapevine virus A

GVA

ATGCAAAGGTTCTTAGGTAGTCTC

GTACTTGTACGATTCCTCCGTCA

327

Grapevine virus B

GVB

TAGTGATGGGTTGTTTGATTACCAT

TGCTCTCTTGGATAGCTCAGATA

355

Rupestris stem pitting-associated  virus

RSPaV

AGCTCTCAAGGCATTAGTAGACT

CCAATGCCCTTTCTACTGACAAC

384

 


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