Table 1. Primer sequences used to detect simultaneously 11 viruses by means of RT-PCR. |
Virus |
Acronym |
Forward primer (5’-3’) |
Reverse primer (5’-3’) |
Product size (bp) |
Arabis Mosaic Virus |
ArMV |
GCCCACGACTTTTCTAATCAAGA |
CTAAACGCCTGTCGGAGTAATAA |
295 |
Grapevine leafroll associated virus 1 |
GLRaV1 |
TTCAGCTTACTGTGGTCTCTATGG |
GCTTCATTTGGAGTCACGAATG |
109 |
Grapevine leafroll associated virus 2 |
GLRaV2 |
TACACCTCTCGCAATGTTTATCAC |
GACCGCTAATCTTCACCGTTAT |
139 |
Grapevine leafroll associated virus 3 |
GLRaV3 |
TAACCACCATGAAGTTCATAGGG |
GCATTAGAAACAGCTTCGAATACGA |
274 |
Grapevine leafroll associated virus 8 |
GLRaV8 |
AGAAGAGAGGGATTGGATTGGAAT |
AGTCTTCTCGGTGGCTTAAATTC |
172 |
Grapevine fanleaf virus |
GFLV |
TTCTTTACTTGCCCTTATGGGC |
CAAAATGCCAGTCGCATTTCTC |
254 |
Grapevine Fleck virus |
GFkV |
TTCACAACACAATCCAGAAGGATAC |
CTCTTCATGAACATGACCGTGG |
262 |
Grapevine rootstock stem lesion associated virus |
GRSLaV |
CAATTGAGTTTTGGTCAGCCTG |
ACGGCAAAAATACCATTCACAC |
317 |
Grapevine virus A |
GVA |
ATGCAAAGGTTCTTAGGTAGTCTC |
GTACTTGTACGATTCCTCCGTCA |
327 |
Grapevine virus B |
GVB |
TAGTGATGGGTTGTTTGATTACCAT |
TGCTCTCTTGGATAGCTCAGATA |
355 |
Rupestris stem pitting-associated virus |
RSPaV |
AGCTCTCAAGGCATTAGTAGACT |
CCAATGCCCTTTCTACTGACAAC |
384 |
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