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Molecular Biology and Genetics |
| Electronic Journal of Biotechnology ISSN: 0717-3458 |
Vol.
12 No. 1, Issue of January 15, 2009 |
| © 2009 by Pontificia Universidad Católica
de Valparaíso -- Chile |
Received May 20, 2008 / Accepted August 7, 2008 |
| DOI: 10.2225/vol12-issue1-fulltext-2 |
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Genetic variability for waxy genes
in Argentinean bread wheat germplasm
Leonardo Sebastián Vanzetti
Grupo de Biotecnología y Recursos Genéticos
INTA EEA Marcos Juárez
Ruta 12 S/N, (2580) Marcos Juárez
Córdoba, Argentina
Tel: 54 3472 425001
Fax: 54 3472 425001
E-mail: lvanzetti@mjuarez.inta.gov.ar
Laura Alicia
Pflüger
Instituto de Recursos Biológicos CIRN-INTA
INTA Castelar
Las Cabañas y Los Reseros, 1712 Castelar
Buenos Aires, Argentina
Tel: 54 11 4621
1819/0840
Fax: 54 11 4621
1819/0840
E-mail: lpfluger@cnia.inta.gov.ar
Marta Rodríguez-Quijano
Unidad de Genética
Departamento de Biotecnología
Escuela Técnica Superior de Ingenieros Agrónomos
Universidad Politécnica 28040
Madrid España
Tel: 34 91 336
5716
Fax: 34 91 543
4879
E-mail: mquijano@bit.etsia.upm.es
José Maria Carrillo
Unidad de Genética
Departamento de Biotecnología
Escuela Técnica Superior de Ingenieros Agrónomos
Universidad Politécnica 28040
Madrid España
Tel: 34 91 336 5716
Fax: 34 91 543
4879
E-mail: josem.carrillo@upm.es
Marcelo Helguera*
Grupo de Biotecnología y Recursos Genéticos
INTA EEA Marcos Juárez
Ruta 12 S/N, (2580) Marcos Juarez
Córdoba, Argentina
Tel: 54 3472 425001
Fax: 54 3472 425001
E-mail: mhelguera@mjuarez.inta.gov.ar
*Corresponding author
Financial support: Grant from the Government of Argentina, FONCyT –
INTA PICTO 12948.
Keywords: characterization,
molecular markers, Triticum aestivum L., starch, waxy genes, wheat.
Abbreviations: |
1D: one dimensional
2D: two
dimensional PCR: polymerase chain reaction
GBSS I:
Granule Bound Starch Synthase I
MAS:
marker assisted selection
SDS-PAGE: Sodium Dodecyl
Sulfate-Polyacrylamide Gel Electrophoresis
SNPs:
single nucleotide polymorphisms |
Amylose and amylopectin are the two polysaccharides
that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound
starch synthase I), also known as waxy protein, is responsible for amylose
synthesis in storage tissues. Decrease of the amylose content in starch has
been associated with the lack of waxy protein(s). In this work, different sets
of PCR markers were used to characterize the genetic variability of waxy loci from 103 Argentinean bread wheat cultivars. For the Wx-A1 locus, Wx-A1a and a novel molecular allele designed Wx-A1g were detected. Wx-B1 locus showed three alleles (Wx-B1a, Wx-B1b, Wx-B1e), and Wx-D1 locus showed only the Wx-D1a allele. Novel
single-locus allele specific markers for Wx-A1b, Wx-B1b and Wx-D1b null alleles were also described. To our best knowledge this is the first study
focused to characterize the genetic variability for waxy genes in bread wheat
cultivars from South America.
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