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Bioinformatics and
Biotechnology |
Electronic Journal of Biotechnology ISSN: 0717-3458 |
Vol.
12 No. 1, Issue of January 15, 2009 |
© 2009 by Pontificia Universidad Católica
de Valparaíso -- Chile |
Received February 26, 2008 / Accepted August 20, 2008 |
DOI: 10.2225/vol12-issue1-fulltext-3 |
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Two LTR retrotransposon elements
within the abscisic acid gene cluster in Botrytis cinerea B05.10, but
not in SAS56
Ming Zhao
Chengdu Institute of
Biology
Chinese Academy of
Sciences
Chengdu 610041, PR China
Graduate University of
Chinese Academy of Sciences
Beijing 100039, China
Tel: 86 028 85219314
Fax: 86 028 85219314
E-mail: zhaoming02292002@yahoo.com.cn
Jin yan Zhou
Chengdu Institute of
Biology
Chinese Academy of
Sciences
Chengdu 610041, PR China
Tel: 86 028 85219314
Fax: 86 028 85219314
E-mail: abath@cib.ac.cn
You jiu Tan
Chengdu Institute of
Biology
Chinese Academy of
Sciences
Chengdu 610041, PR China
Graduate University of
Chinese Academy of Sciences
Beijing 100039, China
Tel: 86 028 85219314
Fax: 86 028 85219314
E-mail: tanyou9@126.com
Wei wei Song
Chengdu Institute of
Biology
Chinese Academy of
Sciences
Chengdu 610041, PR China
Graduate University of
Chinese Academy of Sciences
Beijing 100039, China
Tel: 86 028 85219314
Fax: 86 028 85219314
E-mail: songweiwei426@sohu.com
Zhi dong Li
Chengdu Institute of
Biology
Chinese Academy of
Sciences
Chengdu 610041, PR China
Tel: 86 028 85219314
Fax: 86 028 85219314
E-mail: abath@cib.ac.cn
Hong Tan*
Chengdu Institute of
Biology
Chinese Academy of
Sciences
Chengdu 610041, PR China
Tel: 86 028 85219314
Fax: 86 028 85219314
E-mail: abath@cib.ac.cn
*Corresponding author
Financial support: Key Project of Chinese Academy
of Sciences (No.KSCX2-YW-G016) and the Hi-Tech Research and Development Program
(863) of China (No.2006AA10A213).
Keywords: Botrytis
cinerea, genomic sequence
analysis, in silico, LTR retrotransposon.
Abbreviations: |
ABA: abscisic acid
IN: integrase
domain
LTR: long terminal repeat
PBS: primer binding site
PPT: polypurine tract
RT: reverse transcriptase
domain
TSD: target-site duplication
WGS: Whole Genome Shotgun |
The plant
hormone abscisic acid has huge economic potential and can be applied in
agriculture and forestry for it is considered to be involved in plant
resistance to stresses such as cold, heat, salinity, drought, pathogens and
wounding. Now overproducing strains of Botrytis cinerea are used for
biotechnological production of abscisic acid. An LTR retrotransposon, Boty-aba, and a solo LTR were identified by in silico genomic
sequence analysis, and both were detected within the abscisic acid gene cluster
in B. cinerea B05.10, but not in B. cinerea SAS56. Boty-aba contains a pair of LTRs and two internal genes. The LTRs and the first gene
have features characteristic of Ty3/gypsy LTR retrotransposons. The
second gene is a novel gene, named brtn, which encodes for a protein
(named BRTN) without putative conserved domains. The impressive divergence in
structure of the abscisic acid gene clusters putatively gives new clues to
investigate the divergence in the abscisic acid production yields of different B.
cinerea strains.
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