Table 5. Range and variance analyses of pectinase, proteinase and cellulase in orthogonal experiment. |
Medium |
A |
B |
C |
D |
Pectinase activity (U/g) |
Proteinase activity (U/g) |
Cellulase activity (U/g) |
|||
1 |
1 |
1 |
1 |
1 |
14497 ± 651 |
11713 ± 533 |
2533 ± 112 |
3022 ± 104 |
935 ± 37 |
938 ± 31 |
2 |
1 |
2 |
2 |
2 |
14155 ± 548 |
16006 ± 746 |
3138 ± 143 |
3143 ± 110 |
1139 ± 46 |
1082 ± 43 |
3 |
1 |
3 |
3 |
3 |
13145 ± 602 |
12510 ± 554 |
2614 ± 112 |
2925 ± 98 |
919 ± 41 |
939 ± 40 |
4 |
2 |
1 |
2 |
3 |
14288 ± 673 |
15898 ± 635 |
2819 ± 138 |
2872 ± 79 |
1019 ± 45 |
1058 ± 38 |
5 |
2 |
2 |
3 |
1 |
21168 ± 823 |
20672 ± 594 |
3585 ± 154 |
3761 ± 123 |
1179 ± 42 |
1208 ± 51 |
6 |
2 |
3 |
1 |
2 |
20136 ± 877 |
19916 ± 601 |
3119 ± 162 |
3429 ± 117 |
1074 ± 39 |
1163 ± 44 |
7 |
3 |
1 |
3 |
2 |
15341 ± 647 |
14754 ± 544 |
2334 ± 98 |
2316 ± 89 |
1031 ± 48 |
1088 ± 46 |
8 |
3 |
2 |
1 |
3 |
18230 ± 488 |
17742 ± 823 |
2507 ± 107 |
2719 ± 78 |
1039 ± 41 |
1106 ± 40 |
9 |
3 |
3 |
2 |
1 |
13938 ± 614 |
16500 ± 704 |
3395 ± 99 |
3238 ± 106 |
1070 ± 33 |
1037 ± 42 |
Pectinase |
||||||||||
K1 |
13671 |
14415 |
17039 |
16414 |
|
|
|
|
|
|
K2 |
18680 |
17995 |
15130 |
16718 |
|
|
|
|
|
|
K3 |
16084 |
16024 |
16265 |
15302 |
|
|
|
|
|
|
R |
5009 |
3580 |
1909 |
1416 |
|
|
|
|
|
|
F |
31.3** |
16.1** |
4.6* |
2.8 |
|
|
|
|
|
|
Proteinase |
||||||||||
K1 |
2896 |
2649 |
2888 |
3256 |
|
|
|
|
|
|
K2 |
3264 |
3142 |
3100 |
2913 |
|
|
|
|
|
|
K3 |
2751 |
3120 |
2923 |
2742 |
|
|
|
|
|
|
R |
513 |
493 |
212 |
514 |
|
|
|
|
|
|
F |
14.0** |
15.6** |
2.6 |
13.7** |
|
|
|
|
|
|
Cellulase |
||||||||||
K1 |
992 |
1012 |
1043 |
1061 |
|
|
|
|
|
|
K2 |
1117 |
1126 |
1068 |
1096 |
|
|
|
|
|
|
K3 |
1062 |
1034 |
1061 |
1013 |
|
|
|
|
|
|
R |
125 |
114 |
25 |
83 |
|
|
|
|
|
|
F |
18.6** |
17.3** |
0.8 |
8.2* |
|
|
|
|
|
|
A: H2O (%, w/w); B: load (g, dry weight); C: (NH4)2SO4 (%, w/w); D: KH2PO4 (%, w/w). Each value represents the mean ± standard error; **F>F0.01, *F>F0.05. |
|