Figure 1. PCR products obtained using Wx-A1L/Wx-A1R primers after Hind III digestion. Lanes 1 to 11 are breeding lines/cultivars 1-DHWx12, 2-Komugi Norin, 3-Mariñar, 4-Gamenya, 5-Baguette 10, 6-Buck Brasil, 7-Prointa Molinero, 8-Prointa Puntal, 9-Cronox, 10-Klein Martillo, 11-Prointa Granar, M: 100-pb ladder (Promega), 500-bp fragment is indicated. The black arrowhead indicates the 652-bp fragment from Wx-A1b allele, the grey arrowheads indicate 495-bp and 176-bp fragments from Wx-A1a allele.


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