Figure 3. PCR amplification products obtained using Wx-B1F/Wx-B1R primers. Lanes 1 to 11 are breeding lines/cultivars 1-Biointa 3000, 2-Mariñar, 3-Buck. Pingo, 4-Buck Poncho, 5-Triguero 100, 6-Prointa Bon. Hurón, 7-Prointa. Milenium, 8-Prointa Granar, 9-Gamenya, 10-Cadoux, 11-DHWx12 and lane M is a DNA size standard (100-bp ladder, Biodynamics Corp.). The black arrowhead indicates the 461-bp fragment from Wx-B1a allele, the grey arrowhead indicate 495-bp fragment from Wx-B1e allele and lack of amplification is Wx-B1b allele.