Figure 4. PCR amplification products obtained using Wx-D1L/Wx-D1R primers. Lanes 1 to 11 are breeding lines/cultivars 1-DHWx12, 2-Buck Arriero, 3-Biointa 2001, 4-Klein Gavilán, 5-Baguette 10, 6-Buck Brasil, 7-Prointa Molinero, 8-Prointa Puntal, 9-Cronox, 10-Klein Martillo, 11-Prointa Granar, and lane M: 100-pb ladder (Promega), 500-bp fragment is indicated. The black arrowhead indicates the 930-bp fragment from Wx-D1a allele and the grey arrowhead indicates the 342-bp fragment from Wx-D1b allele.