Figure 5. Best-fit alignment of partial nucleotide sequences (middle region of the Wx-A1 gene) including Wx-A1a (AB019622) and Wx-A1g (DQ431232) alleles from bread wheat. Mismatches are gray-shaded, and exons 5 and 6 regions are in capitals. Locations of #4 F and #4 R primers are also indicated.