Figure 6. Best-fit alignment of partial nucleotide sequences (middle region of the Wx-B1 gene) including Wx-B1a and Wx-B1e alleles from bread wheat (GenBank accessions AB019623 and AY954026). Gaps were introduced to maximize nucleotide alignment and are indicated with dashes, mismatches are gray-shaded, exons are in capital. Annealing sites of #4 L and #4 R primers (McLauchlan et al. 2001) are also indicated.