Table 2. Primer names, sequences, amplified loci and cycling conditions. |
Name |
Sequence |
Amplified loci |
Cycling Conditions |
Wx-A1L |
CCCCAAAGCAAAGCAGGAAAC |
Wx-A1 |
39 cycles of 94ºC 45 sec, 55ºC 30 sec, 72ºC 1 min. |
Wx-A1R |
CGGCGTCGGGTCCATAGATC |
||
Wx-A2L |
CGCAGGGGAAGACGTGGT |
Wx-A1 |
39 cycles of 94ºC 45 sec, 65ºC 40 sec, 72ºC 50 sec. |
Wx-A2R |
CGTTGACGATGCCGGTGATC |
||
Wx-B1L |
CGCAGGGGAAGACGTGGT |
Wx-B1 |
39 cycles of 94ºC 45 sec, 65ºC 40 sec, 72ºC 50 sec |
Wx-B1R |
CGTTGACGATGCCGGTGATG |
||
Wx-D1L |
GCCGACGTGAAGAAGGTGGTG |
Wx-D1 |
39 cycles of 94ºC 45 sec, 55ºC 30 sec, 72ºC 1 min. |
Wx-D1R |
CCCCTTGCGTCATTTGTTGTGT |
||
#4F * |
AAGAGCAACTACCAGT |
Wx-A1, Wx-B1 and Wx-D1 |
(1) Touch down step of 94ºC 1 min, 64ºC to 58ºC (-1ºC/2 cycles) 1 min, 72ºC 1 min. (2) 35 cycles of 94ºC 1 min, 58ºC 1 min, 72ºC 30 sec. |
#4R * |
TCGTACCCGTCGATGAAGTCGA |
*[MgCl2] 3 mM. Other primer combinations [MgCl2] 1.5 mM. |
|