Table 2. Primer names, sequences, amplified loci and cycling conditions.


Name

Sequence

Amplified loci

Cycling Conditions

Wx-A1L

CCCCAAAGCAAAGCAGGAAAC

Wx-A1

39 cycles of 94ºC 45 sec, 55ºC 30 sec, 72ºC 1 min.

Wx-A1R

CGGCGTCGGGTCCATAGATC

Wx-A2L

CGCAGGGGAAGACGTGGT

Wx-A1

39 cycles of 94ºC 45 sec, 65ºC 40 sec, 72ºC 50 sec.

Wx-A2R

CGTTGACGATGCCGGTGATC

Wx-B1L

CGCAGGGGAAGACGTGGT

Wx-B1

39 cycles of 94ºC 45 sec, 65ºC 40 sec, 72ºC 50 sec

Wx-B1R

CGTTGACGATGCCGGTGATG

Wx-D1L

GCCGACGTGAAGAAGGTGGTG

Wx-D1

39 cycles of 94ºC 45 sec, 55ºC 30 sec, 72ºC 1 min.

Wx-D1R

CCCCTTGCGTCATTTGTTGTGT

#4F *

AAGAGCAACTACCAGT

Wx-A1, Wx-B1 and Wx-D1

(1) Touch down step of 94ºC 1 min, 64ºC to 58ºC (-1ºC/2 cycles) 1 min, 72ºC 1 min. (2) 35 cycles of 94ºC 1 min, 58ºC 1 min, 72ºC 30 sec.

#4R *

TCGTACCCGTCGATGAAGTCGA

*[MgCl2] 3 mM. Other primer combinations [MgCl2] 1.5 mM.


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