Table 1 Composition of medium in transformation experiment.

Medium

composition

IDM

MS+500 mg l^-1 LH+1 mg l^-1 Riboflavin+30 g l^-1 Fructose+7 g l^-1 Agar+3 mg l^-1 BA+0.2 mg l^-1 NAA

IDM-Cb

MS+500 mg l^-1 LH+1 mg l^-1 Riboflavin+30 g l^-1 Fructose+7 g l^-1Agar+3 mg l^-1 BA+0.2 mg l^-1 NAA+250 mg l^-1 carbenicillin

IDM-Km

MS+500 mg l^-1 LH+1 mg l^-1 Riboflavin +30 g l^-1 Fructose +7 g l^-1 Agar+3 mg l^-1 BA+0.2 mg l^-1 NAA+250 mg l^-1 carbenicillin+50 mg l^-1 kanamycin