Plant Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 12 No. 2, Issue of April 15, 2009
© 2009 by Pontificia Universidad Católica de Valparaíso -- Chile Received October 23, 2008 / Accepted January 23, 2009
DOI: 10.2225/vol12-issue2-fulltext-11
TECHNICAL NOTE

A rapid and cheap protocol for preparation of PCR templates in peanut

Chuan Tang Wang
 Ocean University of China
Qingdao 266003, P R China

Xiu Zhen Wang
 Shandong Peanut Research Institute
Qingdao 266100, PR China

Yue Yi Tang
 Shandong Peanut Research Institute
Qingdao 266100, PR China

Jian Cheng Zhang
 Shandong Peanut Research Institute
Qingdao 266100, PR China

Shan Lin Yu
 Shandong Peanut Research Institute
Qingdao 266100, PR China

Jian Zhi Xu
 Shandong Peanut Research Institute
Qingdao 266100, PR China

Zhen Min Bao*
Ocean University of China
Qingdao 266003, PR China
Tel: 86 532 8203196
E-mail: zmbao@ouc.edu.cn

*Corresponding author

Financial support: Financial support from the earmarked fund for Modern Agro-industry Technology Research System (MATRS) Peanut Program, MOA, China, China Natural Science Foundation (Grant No. 30300224), 863 New and High Technology Project (Grant No. 2002CCC03200, 2006AA10A114), New and High Technology Innovation Foundation of Shandong Academy of Agricultural Sciences (Grant No. 2006 YCX013), and Young Scientists Foundation of Shandong Academy of Agricultural Sciences (Grant No.2007YQN007) is gratefully acknowledged. We are in debt to Prof Rong Hua Tang for his generosity in providing wild peanuts.

Keywords: DNA extraction, groundnut, PCR, peanut.

Abbreviations:

GXAS: Gaungxi academy of agricultural sciences
ITS: internal transcribed spacer
MAS: marker-assisted selection
PCR: polymerase chain reaction
PVP: polyvinylpyrrolidone
SCAR: sequence-characterized region
SSR: simple sequence repeat
Abstract   Full Text

This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.
 
Supported by UNESCO / MIRCEN network