A rapid and cheap protocol for preparation of PCR templates in peanut
Xiu Zhen Wang
Yue Yi Tang
Jian Cheng Zhang
Shan Lin Yu
Jian Zhi Xu
Financial support: Financial support from the earmarked fund for Modern Agro-industry Technology Research System (MATRS) Peanut Program, MOA, China, China Natural Science Foundation (Grant No. 30300224), 863 New and High Technology Project (Grant No. 2002CCC03200, 2006AA10A114), New and High Technology Innovation Foundation of Shandong Academy of Agricultural Sciences (Grant No. 2006 YCX013), and Young Scientists Foundation of Shandong Academy of Agricultural Sciences (Grant No.2007YQN007) is gratefully acknowledged. We are in debt to Prof Rong Hua Tang for his generosity in providing wild peanuts.
Keywords: DNA extraction, groundnut, PCR, peanut.
This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.