Molecular Biology and Genetics
  

Microbial Biotechnology
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 12 No. 2, Issue of April 15, 2009
© 2009 by Pontificia Universidad Católica de Valparaíso -- Chile Received June 5, 2008 / Accepted November 3, 2008
DOI: 10.2225/vol12-issue2-fulltext-5
TECHNICAL NOTE

Genetic and physical map of broad host range cosmid pRG930cm

Andrew Chibeu#
 Laboratory of Microbial Interactions
Department of Cellular and Molecular Interactions
Flanders Interuniversity Institute of Biotechnology
Vrije Universiteit Brussel
Pleinlaan 2, B-1050
Brussels, Belgium

Rob Lavigne*
 Division of Gene Technology
Katholieke Universiteit Leuven
Kasteelpark Arenberg 21
Leuven, B-3001, Belgium
Fax: 32 016 32 19 65
E-mail: rob.lavigne@biw.kuleuven.be

Wesley Mattheus
Division of Gene Technology
Katholieke Universiteit Leuven
Kasteelpark Arenberg 21
Leuven, B-3001, Belgium 

Sandra Matthijs
 Laboratory of Microbial Interactions
Department of Cellular and Molecular Interactions
Flanders Interuniversity Institute of Biotechnology
Vrije Universiteit Brussel
Pleinlaan 2, B-1050
Brussels, Belgium 

Pierre Cornelis
 Laboratory of Microbial Interactions
Department of Cellular and Molecular Interactions
Flanders Interuniversity Institute of Biotechnology
Vrije Universiteit Brussel
Pleinlaan 2, B-1050
Brussels, Belgium

Guido Volckaert
 Division of Gene Technology
Katholieke Universiteit Leuven
Kasteelpark Arenberg 21
Leuven, B-300, Belgium

*Corresponding author

Financial support: AC was initially supported by a joint grant of the laboratories involved and currently holds an IRO scholarship from KU Leuven. RL holds a postdoctoral fellowship from the FWO (Fonds voor Wetenschappelijk Onderzoek), Vlaanderen.

Keywords: cosmid, pRG930cm, Pseudomonas, sequencing.

Present address: #Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.

Abbreviations:

CmR: chloramphenicol resistance cassette
IncPα: incompatibility group P alpha
MCS: multiple cloning site
ORF: open reading frame
Abstract   Full Text

We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.
Supported by UNESCO / MIRCEN network