Table 1. Peanut materials used for primary evaluation of the protocol.

No.

Genotype

Leaflet

Primer pair

Treatment

 1

 I8B4

 immature

 TBPfex1, TBPrex1

 40 µl NaOH

 2

 L-1

 immature

 TBPfex1, TBPrex1

 40 µl NaOH

 3

 Luhua 14

 immature

 TBPfex1, TBPrex1

 40 µl NaOH

 4

 6-33

 immature

 TBPfex1, TBPrex1

 40 µl NaOH

 5

 m5

 immature

 TBPfex1, TBPrex1

 40 µl NaOH

 6

 I8B4

 immature

 TBPfin2, TBPrin2

 40 µl NaOH

 7

 L-1

 immature

 TBPfin2, TBPrin2

 40 µl NaOH

 8

 Luhua14

 immature

 TBPfin2, TBPrin2

 40 µl NaOH

 9

 6-33D3

 immature

 TBPfin2, TBPrin2

 40 µl NaOH

 10

 L7-1

 immature

 a, b

 40 µl NaOH

 11

 m5

 immature

 a, b

 40 µl NaOH

 12

 L7-1

 field-grown

 a, b

 20 µl NaOH

 13

 L7-1

 field-grown

 a, b

 40 µl NaOH

 14

 L7-1

 field-grown

 a, b

 60 µl NaOH

 15

 m5

 field-grown

 a, b

 20 µl NaOH

 16

 m5

 field-grown

 a, b

 40 µl NaOH

 17

 m5

 field-grown

 a, b

 60 µl NaOH