Figure 2. Embryogenic callus morphotypes and regeneration:
(A) Granular and yellowish pro-embryogenic callus cultivated on Chée and Pool (1987) supplemented with 9 µM of 2,4-D and 11.35 µM of TDZ under dark conditions (Bar: 1 mm).
(B) Pro-embryogenic callus observed since the third subculture (pale-yellow colour) (Bar: 1 mm).
(C) Spontaneous mature embryos observed after the fifth subculture (Bar: 1 mm).
(D) Soft and necrotic tissues observed after the fifth subculture (Bar: 1 mm).
(E) Asynchronous embryogenic callus, cultivated on Chée and Pool (1987) with 4.52 µM of 2,4-D and 2.89 µM of TDZ under 16 hrs photoperiod, exhibiting somatic embryos at different developmental stages (Bar: 1 mm).
(F) Mass production of somatic embryos on Chée and Pool (1987) with 4.52 µM of 2,4-D and 2.89 µM of TDZ under 16 hrs photoperiod.
(G) Regenerated plantlets on phytohormone-free MS medium.