Table 4. Mean number of mature somatic embryos derived from long-term maintained calluses within 12, 24, 36 and 48 months.


Cultivars

Mean number of mature somatic embryos
within a proliferation period of

12 months

24 months

36 months

48 months

 ‘Asli’ (*)

 234.33 ± 11.26 b

 245.33 ± 12.77  b

 282 ± 32.6 a

 192 ± 14.11 c

 ‘Kahli Kerkennah’ (*)

 318.17 ± 12.92 a

 329.66 ± 17.77 a

 263.67 ± 26.01 b

 230.83 ± 19.02 c

 ‘Muscat Raf-raf’ (NS)

 220.33 ± 7.15

 208.33 ± 14.18

 209.33 ± 12.14

 211.66 ± 3.37

 ‘Arich Dressé’(*)

 218.5 ± 11.33 b

 248.16 ± 14.49 a

 189.5 ± 12 c

 240.66 ± 12.3 a

 ‘Turky’ (*)

 260.5 ± 11.58 a

 239.16 ± 17.97 b

 265.0 ± 11.51 a 

 211.5 ± 7.49 c

 ‘Kahli Sfax’(NS)

 232.83 ± 7.4

 243.5 ± 4.98

 241.33 ± 5.57

 245.83 ± 12.81

 ‘Khamri Tozeur’(NS)

 253.83 ± 10.5

 265.5 ± 8

 272.16 ± 9.73

 267.5 ± 8.99

 ‘Arich Ahmar’(NS)

 242.7 ± 10.3

 252.7 ± 6.3

 266.7 ± 10.5

 260.1 ± 11.6

Somatic embryos were counted on embryogenic masses (50 mg fresh weight), in each cultivar.
The values are the means of 6 replicates per cultivar.
For each cultivar, means followed by different letters are significantly different at P < 0.05 according to Duncan’s multiple range tests.
Abbreviations: NS: Not Significant; *: Significant.


Supported by UNESCO / MIRCEN network