Table 1. ORFs encoded on the cosmid vector pRG930cm.

Position

Gene name

Function/characteristic

Source

 1570-3357

 traI

 DNA relaxase

 (Thorsted et al. 1998)

 3354-3728

 traJ

 OriT-recognizing protein

 (Fürste et al. 1989; Ziegelin et al. 1989)

 4506-5336

 kfrA

 Transcription regulation

 (Jagura-Burdzy et al. 1992)

 6108-7283

 parB

 pVS1 replication protein ParB

 (Austin and Abeles, 1983)

 7374-7589

 parG

 pVS1 partioning protein Par G

 (Hayes, 2000)

 7610-8239

 parA

 Partitioning protein ParA

 (Austin and Abeles, 1983)

 8326-8541

 ORF 3

 Hypothetical protein

 8538-9224

 Resolvase

 (Swinfield et al. 1991)

 9560-9790

 ORF 1

 Bacteriophage phi 80 unknown gene

 9952-10680

 ORF 2

 Bacteriophage phi 80 unknown gene

 11288-12301

 IntI

 Integrase

 (Esposito and Scocca, 1997)

 12300-13241

 aadA

 streptomycin/spectinomycin adenyltransferase

 (Goldschmidt-Clermont, 1991)

 13702-14361

 cat

 Chloramphenicol acetyltransferase

 (Shaw, 1983)

 15161-15529

 mob

 Mob-like protein

 15528-15752

 rom

 Rom/Rop protein. RNA I inhibition modulator protein

 (Cesareni et al. 1982)

 16178-16776

 ColE1 ori

 (Hashimoto-Gotoh and Timmis, 1981)