Molecular Biology and Genetics
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 12 No. 3, Issue of July 15, 2009
© 2009 by Pontificia Universidad Católica de Valparaíso -- Chile Received September 26, 2008 / Accepted April 13, 2009
DOI: 10.2225/vol12-issue3-fulltext-10
RESEARCH ARTICLE

Analysis of diversity among six populations of Colombian mango (Mangifera indica L. cvar. Hilacha) using RAPDs markers

Marcela Díaz-Matallana
 Unidad de Biotecnología Vegetal
Departamento de Biología
Facultad de Ciencias
Pontificia Unidad Javeriana
Bogotá, D.C., Colombia 

Ingrid Schuler-García*
 Departamento de Biología
Facultad de Ciencias
Pontificia Universidad Javeriana
Cra. 7 No. 43-82 Edificio Carlos Ortíz (52), 5o piso
Bogotá, D.C. , Colombia
E-mail: ischuler@javeriana.edu.co 

Manuel Ruiz-García
 Unidad de Genética y Biología del Desarrollo
Departamento de Biología
Facultad de Ciencias
Pontificia Universidad Javeriana
Bogotá, D.C., Colombia 

Elizabeth Hodson de Jaramillo
 Unidad de Biotecnología Vegetal
Departamento de Biología
Facultad de Ciencias
Pontificia Universidad Javeriana
Bogotá, D.C., Colombia

*Corresponding author

Financial Support: Research Programme “Young Researchers and Innovators” (2003-2005), funded by COLCIENCIAS (Instituto Colombiano Francisco José de Caldas para el Desarrollo de la Ciencia y Tecnología) and Pontificia Universidad Javeriana, Bogotá, D.C.

Keywords: genetic variability, mango Hilacha, organoleptic quality, RAPDs.

Abbreviations:

AFLP: amplified fragment length polymorphism
IDEA: Fundación Instituto de Estudios Avanzados (Venezuela)
ISTR: inverse sequence tagged repeat
NTSYSpc: numerical taxonomy and multivariate analysis system ver. 2.02j
POPGENE: population genetic analysis ver. 1.32
RAPD: random amplified polymorphic DNA
RFLP: restriction fragment length polymorphism
SSRs: simple sequence repeats
STRs: short tandem repeats
TFPGA: tools for population genetic analyses ver.1.3
UPGMA: unweighted pair group method with arithmetic averages
VNTRs: variable number of tandem repeats
Abstract   Full Text

Currently in Colombia, there are only records of morph-agronomic characterizations of Mangifera indica cvar. Hilacha; molecular studies on this mango variety have not been carried out. The aim of this work was to identify the genetic diversity of six populations of mango Hilacha by RAPDs markers, as a fundamental base for breeding programs, conservation and selection of promissory materials for the fruit industry at the national level. From 60 primers evaluated in the populations, five primers were selected and were launched in the six populations. Polymorphic bands of RAPDs were transformed into binary matrices, which were then processed with NTSYS-PC, POPGENE and TFPGA softwares. The overall genetic diversity, HT = 0.468 + 0.0016, is very similar to the average subpopulation genetic diversity, HS = 0.4431 + 0.0024, which revealed a small genetic differentiation among the mango Hilacha populations studied (GST = 0.0532). This means that each population contained in average 95 % of the total genetic diversity found in the global population analyzed. Considerable gene flow between populations (Nm = 9) was found. Finally, we recommend studying the genetic diversity of mango Hilacha populations with other molecular markers to complement the information obtained and to find similarities or differences with the results presented herein.
Supported by UNESCO / MIRCEN network