Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 12 No. 3, Issue of July 15, 2009
© 2009 by Pontificia Universidad Católica de Valparaíso -- Chile Received September 3, 2008 / Accepted March 30, 2009
DOI: 10.2225/vol12-issue3-fulltext-8

Micro-scale surface-patterning influences biofilm formation

Guimel M. Kappell
Department of Biology
The University of Texas at Arlington
Arlington Texas, 76019, USA

James P. Grover
Department of Biology
The University of Texas at Arlington
Arlington Texas, 76019, USA 

Thomas H. Chrzanowski*
Department of Biology
The University of Texas at Arlington
PO Box 19498, Arlington Texas, 76019, USA
Tel: 817 272 2404
Fax: 817 272 2855

*Corresponding author

Keywords: indwelling medical devices, infections, surface attachment.


EPS: extra-polymeric substances
SiE: silicone elastomer
TSB: tryptic soy broth

Abstract   Full Text

The formation of biofilms on indwelling/implanted medical devices is a common problem. One of the approaches used to prevent biofilm formation on medical devices is to inhibit bacterial attachment by modification of the synthetic polymers used to fabricate the device. In this work, we assessed how micro-scale features (patterns) imprinted onto the surface of silicone elastomer similar to that used for medical applications influenced biofilm formation by Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Patterns were transferred from a multi-patterned oxidized silicon-wafer master-template to silicone elastomer. Features consisted of bars, squares, and circles each extending 0.51 µm above the surface. Feature sizes ranged between 1.78 and 22.25 µm. Distances separating features ranged between 0.26 and 17.35 µm. Bacterial biofilm formation on discs cut from imprinted silicone elastomer was assessed by direct microscopic observation and quantified as the surface area covered by biofilm. Unpatterned silicone elastomer served as a control. Several of the micro-scale patterns imprinted into the silicone elastomer significantly reduced biofilm formation by each bacterium and interrupted biofilm continuity. Although there were differences in detail among strains, bacteria tended to attach in the area between features more than to the surface of the feature itself.

Supported by UNESCO / MIRCEN network