Marine Biotechnology

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 12 No. 3, Issue of July 15, 2009
© 2009 by Pontificia Universidad Católica de Valparaíso -- Chile Received January 20, 2009 / Accepted May 8, 2009
DOI: 10.2225/vol12-issue3-fulltext-9
SHORT COMMUNICATION

Microsatellites within genes and ESTs of the Pacific oyster Crassostrea gigas and their transferability in five other Crassostrea species

Qi Li*
Fisheries College
Ocean University of China
Qingdao 266003
P.R. China
Tel: 86 532 82031622
Fax: 86 532 82032773
E-mail: qili66@ouc.edu.cn 

Shikai Liu
Fisheries College
Ocean University of China
Qingdao 266003
P.R. China

Lingfeng Kong
Fisheries College
Ocean University of China
Qingdao 266003
P.R. China

*Corresponding author

Financial support: National High Technology Research and Development Program (2006AA10A409) and Ministry of Education of China (707041).

Keywords: indwelling medical devices, infections, surface attachment.

Abbreviations:

AFLP: amplified fragment length polymorphism
EST: expressed sequence tags
He: expected heterozygosity
Ho: observed heterozygosity
HWE: Hardy-Weinberg equilibrium
PCR: polymerase chain reaction
SSR: simple sequence repeat

Abstract   Full Text

We developed 15 novel polymorphic microsatellites for the Pacific oyster Crassostrea gigas by screening genes and expressed sequence tags (ESTs) found in GenBank. The number of alleles per locus ranged from two to 24 with an average of 8.7, and the values of observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.026 to 0.750 and from 0.120 to 0.947, respectively. No significant pairwise linkage disequilibrium was detected among loci and eight loci conformed to Hardy-Weinberg equilibrium. Transferability of the markers was examined on five other Crassostrea species and all the markers were amplified successfully in at least one species. These new microsatellites should be useful for population genetics, parentage analysis and genome mapping studies of C. gigas and closely related species. The nine markers identified from known genes are expected to be especially valuable for comparative mapping as type I markers.

Supported by UNESCO / MIRCEN network