Table 3. DNA sequence alignment result showing base substitution and base insertion in synthetic huEPO B gene. The underlined bases are the bases that were substituted with the bases underneath them. The dash (-) indicates the position of the inserted base with the base underneath it.

Location (bp)

Alignment analysis

53

TCTCCT-GTCCCT

T

121

CC-GAGTCC

                                C

144, 157

TACCTCTTGGAGGCCAAGGAGGCCGAGAAT

                   C                                 A

241-243

ATGCCTGGAAG

                             CGG

287, 294

GTCTGGCAGGGCCTGGCCC

                                T                C

300

TG-CTGTCG

                                G

341

TTGGTCAACTCTTCC

C