Figure 1. Calculated (a) and observed (b) HaeIII RFLP patterns that occurred among 237 cloned archaeal 16S rRNA fragments from sludge taken from bottom and middle of the bioreactor and the recycling tank. Fragment sizes were inferred from DNA sequences of representative clones. M: 100 kb molecular mass marker (Fermentas, Hanover, Maryland, USA); RFLP patterns were labeled ‘I’; SamaliEB, II; Samali15’ and M. concilii (M59146).