Figure 3.
Recombinant expression of MtsPLA2.
(a) SDS-poly acrylamide gel
electrophoresis (PAGE) analysis of expressed protein. Lane M, protein markers.
Lane 1, cells with only the pET28a; lane 2, uninduced cells with the expression
construct; lane 3, induced cells with the expression construct; lane 4, Ni-NTA
purified MtsPLA2; lane
5, refolded MtsPLA2.
(b) Western blot analysis.◄ indicates
the MtsPLA2. |