Figure 3. Recombinant expression of MtsPLA2.
(a) SDS-poly acrylamide gel electrophoresis (PAGE) analysis of expressed protein. Lane M, protein markers. Lane 1, cells with only the pET28a; lane 2, uninduced cells with the expression construct; lane 3, induced cells with the expression construct; lane 4, Ni-NTA purified MtsPLA2; lane 5, refolded MtsPLA2.
(b) Western blot analysis.◄ indicates the MtsPLA2.


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