Plant Biotechnology
  Biotechnology Industry
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol. 12 No. 4, Issue of October 15, 2009
© 2009 by Pontificia Universidad Católica de Valparaíso -- Chile Received July 10, 2008 / Accepted September 21, 2009
DOI: 10.2225/vol12-issue4-fulltext-10
RESEARCH ARTICLE

Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration

Kiriaki Chatzidimitriadou
School of Agriculture
Aristotle University of Thessaloniki
Thessaloniki, Greece 

Irini Nianiou-Obeidat
School of Agriculture,
Aristotle University of Thessaloniki
Thessaloniki, Greece 

Panagiotis Madesis
School of Agriculture,
Aristotle University of Thessaloniki
Thessaloniki, Greece 

Rafael  Perl-Treves
Department of Life Sciences
Bar-Ilan University
Ramat Gan, Israel

Athanasios Tsaftaris*
School of Agriculture
Aristotle University of Thessaloniki
Insitute of Agrobiotechnology
Thessaloniki, Greece
E-mail: tsaft@agro.auth.gr

*Corresponding author

Financial support: Grant from Greek Secretary for Research and Technology to A.S.T.

Keywords: Capsicum annum L., drought stress, MV, regeneration, SOD.

Abbreviations:

CAT:catalase
MV: methyl viologen
RWC: relative water content
ROS: reactive oxygen species
SOD: superoxide dismutase

Abstract   Full Text

The objective of this work was to study the stress tolerance and regeneration capability of transgenic pepper plants carrying a sod gene, encoding a tomato chloroplast-localized Cu/Zn SOD protein. The expression of the sod gene was confirmed by enzymatic staining following polyacrylamide gel electrophoresis (PAGE), revealing a ‘novel’ band, which could represent a heterodimeric enzyme. Transgenic T1 and T2 progeny plants were exposed to different oxidative stresses including Methyl viologen (MV) and drought and found to have an increased resistance to oxidative damage. Furthermore, the SOD carrying transgenic pepper plants showed increased levels of regeneration efficiency compared to the wild type pepper plants. Pepper is a recalcitrant species in terms of its in vitro regeneration ability but it could be extremely useful for the development of pharmaceuticals. This approach enables the extent use of pepper for genetic transformation and the production of high valuable products in plants particularly the large fruit varieties.

Supported by UNESCO / MIRCEN network